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The Effect of Cryodiluent Type And Dilution Ratios On Post-Thaw Motility
Of Giant Grouper (Epinephelus Lanceolatus) Sperm
Wan Nurizzati binti W Idris
Supervisor: Dr Ivan Koh Chong Chu
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences (Aquaculture)
Sperm cryopreservation helps to preserve valuable strains of male broodstocks which
is important for seed production of giant grouper (Epinephelus lanceolatus). In this
study, we investigated the effects of various cryodiluents [10-20%; Dimethyl
sulphoxide (DMSO), Propylene Glycol, Methanol], dilution ratios (1:1, 1:19 and 1:49)
and toxicity (till 30 minutes) on sperm cryopreservation of giant grouper.
Cryopreservation was done by cooling the sperm samples at 7.5 cm height above
liquid nitrogen (LN) vapor before immersion in LN after 3 minutes. Toxicity was
determined by observing samples at set intervals after dilution in 10% DMSO. Our
results show that 15% DMSO was the best cryoprotectant and there was no
significance differences in post-thaw motility at all dilution ratios. Also, 10% DMSO
was not toxic to sperm up to 30 minutes. In conclusion, we have developed a speciesspecific
cryopreservation protocol for giant grouper sperm that will be useful for giant
grouper seed production.
544 | UMT UNDERGRADUATE RESEARCH DAY 2018