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Bioluminescent Bacteria as Potential Biosensor for the Detection of<br />

Nitrogen and Phosphorous Pollution<br />

Nur Suzana binti Mohd Zain<br />

Supervisor: Assoc. Prof. Dr. Muhd Danish Daniel bin Abdullah<br />

Bachelor of Applied Science (Fisheries)<br />

School of Fisheries and Aquaculture Sciences<br />

Bioluminescent bacteria are organism that have unique characteristics. Currently,<br />

bioluminescent bacteria have been used widely as biosensor in the detection of the<br />

pollution. In this study, bioluminescent bacteria isolated from squids were used as<br />

biosensor for the detection of nutrient pollution that are commonly associated with<br />

aquaculture environment such as NaNO3, NaNO2, KH2PO4 and (NH4)2SO4. This study<br />

also aims to observe the response of the bioluminescent bacteria towards the<br />

pollutants at different concentrations. The bacteria were isolated from the body<br />

surface of squid and identified as Photobacterium damselae (93% similarity). Light<br />

intensity shows consistent decrease with increased toxicity of NaNO3 and NaNO2,<br />

consistent increase with increased toxicity of KH2PO4 and does not consistent decrease<br />

with increasing concentration of (NH4)2SO4. The bioluminescent bacteria are<br />

potentially useful as pollutant biosensor especially for NaNO3 and NaNO2 but not for<br />

KH2PO4 and (NH4)2SO4. Further study is needed in the future especially involving field<br />

testing.<br />

626 | UMT UNDERGRADUATE RESEARCH DAY 2018

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