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The Effects of Passiflora Foetida Crude Extract On High Glucose-Induced
Oxidative Stress in 3t3 Fibroblast Cells
Nuraini Binti Mohamed Zuki
Supervisor: Dr. Maziah Binti Ghazaly
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Type-2 diabetes mellitus is characterised as having high glucose level in blood.
Currently, traditional herbal medicines were still preferred to be used to treat diabetes.
Passiflora foetida (P. foetida) was chosen in this study because its metabolic cellular
signalling mechanisms in cells are unknown. This study is to investigate its effects on
cell viability, cell migration and cellular reactive oxygen species (ROS) profile on cells
treated with high glucose detected by MTT assay, migration assay and 96-well plate
dihydroethidium (DHE) fluorescent assay respectively. It was found that the extract
was non-toxic to cells, able to increase 50% of cell migration and there is a reduction
trend of ROS level in 3T3 fibroblast cells following treatment. Therefore, the extract
may have potentials to act as an anti-diabetic treatment for its effectiveness. Further
study need to be done to elucidate further on the cellular signalling mechanisms for
effective drug discovery in the future.
Keywords: Passiflora foetida, cellular signalling, high glucose, reactive oxygen species
378 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018