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Catalase, Ascorbate Peroxidase and Guaiacol Peroxidase Enzymatic
Activities of Chlorella Vulgaris in Different Growth Phases
Nur Maisarah Athirah Binti Khazali
Supervisor: Dr. Norhayati binti Yusuf
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Chlorella vulgaris is considered as a potential antioxidant as they enable to generate
enzymatic antioxidants against oxidation from reactive oxygen species (ROS). This
study was conducted to determine the growth curve as well as compare catalase,
ascorbate peroxidase and guaiacol peroxidase specific activities in different growth
phases of C. vulgaris. C.vulgaris was cultured in Guillard f/2 medium at 25±2°C under
laboratory condition. The results indicated catalase production was higher at
exponential phase 1.50 ±0.08 units/mg protein, whereas ascorbate peroxidase and
guaiacol peroxidase were higher at lag phase 37.13 ±4.93 units/mg protein and 1.31
±0.03 units/mg protein respectively. C.vulgaris produced highest catalase, ascorabate
peroxidase and guaiacol peroxidase enzymatic activities in exponential and lag phase.
Production of enzymatic activities in growth phases can be used as guideline to
produce massive enzymatic antioxidants, which commercialized in the food and
pharmaceutical industry. In addition, further studies also are required to have better
understanding of C.vulgaris.
328 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018