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Synthesis and Characterization of Alkanethiol Compound as a Potential
Recognition Layer in Electrochemical DNA Sensor
Asilah Binti Mohammad Yatim
Supervisor: Dr. Hanis Bt Mohd Yusoff
Bachelor of Science (Chemical Sciences)
School of Fundamental Science
Electrochemical DNA sensor is a technique that can identify and diagnose various
diseases due to its highly sensitive and fast response. This study was conducted to
find a suitable chain length of alkanethiol to be applied as potential linker on the gold
substrate through the self-assembled monolayer (SAMs). Two potential linkers,
hexanethiol and heptanethiol were successfully synthesized and characterized using
Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR)
spectroscopy. Cyclic Voltammetry (CV) was used to study the self-assembled of
difference linkers on the gold electrode and the immobilization of DNA target gene.
The working electrode was prepared by depositing gold substrate onto indium-tin
oxide (ITO) glass by using autocoater. Each measurement of modified gold electrode
was performed in the standard parameter before being hybridized with the DNA target
gene. The increase or decrease in the electron transfer for both linkers were observed
as CV peak current and potential change.
406 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018