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Chemical study of Malaysian stingless bee propolis: Geniotrigona thoracica<br />

Nur Husna Ashikin Binti Mohamad Noor<br />

Supervisor: Dr. Ahmad Nazif bin Aziz<br />

Bachelor of Science (Chemical Sciences)<br />

School of Fundamental Science<br />

Propolis has been studied for their biological properties worldwide. However, the study<br />

on its chemical composition is very rare, especially on the Malaysian stingless bee<br />

propolis. In this study, the propolis of Geniotrigona thoracica, a Malaysian stingless<br />

bee was investigated for its chemical constituents. Phytochemical screening showed<br />

the presence of phenols, tannins, flavonoids, steroids, terpenoids, alkaloids and<br />

glycosides in the propolis. The methanol, hexane and a fraction from hexane extract<br />

namely GTBH3 were screened for cytotoxicity against MCF-7 breast cancer and SKUT-<br />

1 fibroid cancer cell lines. Fraction GTBH3 and methanol extract showed highly<br />

cytotoxicity against SKUT-1 with IC50 of 2 μg/mL and 4 μg/mL respectively while<br />

GTBH3 showed most highly cytotoxicity against MCF-7 with IC50 of 10 μg/mL. The<br />

hexane extract was selected for isolation and purification using Column<br />

Chromatography and Thin Layer Chromatography. Two compounds were successfully<br />

isolated and analyzed using Infrared, Nuclear Magnetic Resonance, Ultraviolet and<br />

Mass Spectrometry.<br />

440 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018

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