13.05.2018 Views

merged

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Secondary Metabolites from the Fruits of Coastal Plant Vitex Rotundifoila<br />

and Antioxidant Activity<br />

Akmar Faris Bin Mohd. Hisam<br />

Supervisor: Dr. Noor Wini Binti Mazlan<br />

Bachelor of Science (Analytical and Environmental Chemistry)<br />

School of Marine and Environmental Science<br />

Universiti Malaysia Terengganu<br />

Vitex rotundifolia is commonly known as “beach Vitex”, a coastal plant that form floorcoverings<br />

grows near coastal area. The secondary metabolites from the fruits of V.<br />

rotundifolia possess potential biological activities. In this study, the fruits of V.<br />

rotundifolia were collected in Penarik coastal area, Setiu. The fruits of V. rotundifolia<br />

were extracted using methanol to obtain a methanolic crude extract. The methanolic<br />

crude extract was fractionated using vacuum liquid chromatography (VLC) with<br />

different solvent polarity to give 24 fractions. The fraction 6 was further isolated to<br />

give vitexicarpin by using chromatography techniques and the molecule structure was<br />

identified using 1D and 2D-nuclear magnetic resonance (NMR) including COSY and<br />

HMBC NMR. Seven out of 24 fractions were evaluated for antioxidant using DPPH free<br />

radical scavenging assays. The fraction 18 and 24 showed potent antioxidant activity<br />

inhibition concentration at 50 % (IC50) value of 0.375 mg/mL.<br />

1931 | UMT UNDERGRADUATE RESEARCH DAY 2018

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!