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Effect of temperature and irradiance on growth of Chlorella sp using
aquaculture wastewater
Norfazilah Binti Salim
Supervisor: Dr Emienour Muzalina binti Mustafa
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Microalgae have ability to grow in aquaculture wastewater (AWW) by removing the
excess nutrient in the wastewater and the variation of temperature and irradiance can
influenced the growth of microalgae. The objective of this study is to monitor the
growth of Chorella sp. in AWW under different temperature and irradiance. In this
study, Chlorella sp were cultured in different concentration of AWW (0,50% &100%
AWW) under different temperature and irradiance conditions (indoor & outdoor) for
12 days. The growth of Chorella sp. were measured based on optical density at 620nm
(OD620nm), chlorophyll a, carotenoid, algae cell count & dry weight content on day
0, 2, 4,6,8,10 & 12. The water quality parameter (ammonia & phosphate) were
measured on day 0 & 12. Results shows that the Chlorophyll a (305.2161 mg/l Chl a)
and carotenoid (3.5062 ug/ml carotenoid) were highest in cultures grown in 50%
AWW on day 8 while the dry weight (217.33 mg/l) & OD620nm (0.614) obtained were
highest on day 12. The nutrient content in cultures grown in AWW reduced up to
77.72% (50% AWW) for ammonia & 60.30% (100%AWW) for phosphate. In
conclusion, we found that Chorella sp. can grow well in 50% AWW > 100% AWW >
BBM Medium (control) under indoor condition (low temperature & irradiance).
512 | UMT UNDERGRADUATE RESEARCH DAY 2018