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The Cytotoxicity Effect and the Mode of Cell Death of Iotrochota Baculifera
Extract on Human Cervical Cancer Cell Line (HELA)
Ambrose A/L Louise
Supervisor: Dr.Vigneswari A/P Sevakumaran
Co- Supervisor: Professor Dr. Tengku Sifzizul Bin Tengku Muhammad
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Sponges are animals that are sessile and filter feeders in which has the potential to
be agents for pharmaceutical applications, including anticancer. In this study, sponge
species of Iotrochota baculifera extract was studied for its cytotoxicity effects and the
mode of cell death on human cervical cancer cell line (HeLa). MTS Assay was used to
determine the cytotoxicity effects, Annexin Vanadium & Propidium Iodide Assay were
done in detecting the induction of apoptosis. Results showed that I. baculifera extract
can kill 50% of the HeLa cells with the Inhibitory Concentration (IC50) of 7.7 µg/mL
and can induce cells death via apoptosis. Due to the profound cytotoxic and apoptotic
activities, further studies can be done on the extract to determine the specific
compound involved in the mechanism.
403 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018