merged

The effect of streptomycin supplementation on the quality of cold stored
malaysian mahseer (Tor tambroides) sperm
Muhammad Nursyahidin Bin Ampandi
Supervisor: Dr Ivan Koh Chong Chu
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences (Aquaculture)
This study aims to determine the effect of different concentration of streptomycin
on the motility of cold stored Malaysian Mahseer (Tor tambroides) sperm. Sperm
was collected from mature males (n=4) and diluted with Artificial Seminal plasma
(ASP) in 1.5ml tubes. Antibiotic was added into samples at different concentrations
(0, 1000, 2000, 3000 ug/ml) and stored at 2-4˚C. The sperm quality parameters
of percentage motility and duration of motility were then measured at 0, 6, 12, 24,
48, 72 hours after storage until no motility was detected. The addition of antibiotics
significantly increased, duration of sperm motility compared to control. The results
showed that sperm motility was significantly higher with antibiotic dosages of 1000
to 3000 ug/ml, with motility till 120h. In conclusion, the results show that
streptomycin with concentration of 1000-3000 ug/ml improves the quality of
Malaysian Mahseer sperm during cold storage.
505 | UMT UNDERGRADUATE RESEARCH DAY 2018