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Identification and Molecular Analysis of Bacteria Isolated From Contact
Lens Solutions
Nuryasmin Ezzaty Bt Anapil@Salleh
Supervisor: Dr. Siti Nor Khadijah binti Addis
Bachelor of Science (Biological Science)
School of Fundamental Science
Contact lens solution can be contaminated by various types of bacteria due to the
wrong handling. The bacteria transferred into the contact lens solution via indirect
contact. A contaminated contact lens solution can caused a severe eye infection. Thus,
the aim of this study is to identify the bacteria isolated from contact lens solution by
performing Polymerase Chain Reaction to amplify the bacteria 16s rDNA gene. From
previous study, four bacteria samples identified as Gram-positive bacteria and another
five bacteria samples were Gram-negative bacteria and these bacteria were
antimicrobial resistance. Staphylococcus sp. is could be identified among the Grampositive
bacteria that frequently cause conjunctiva. Meanwhile, Serratia sp. could be
possible identified among Gram-negative bacteria that can cause keratitis. In this
study, the eight bacteria samples successfully underwent 16s rDNA gene amplification.
This study is important to provide information to improve the understanding in the
association of bacteria with eye infection among contact lens wearers in Malaysia.
342 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018