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Effect of Extraction Solvent on Total Phenol Content, Total Flavonoid Content<br />

and Antioxidant Activity of Piper sarmentosum (Daun Kaduk)<br />

Wan Nursyahirah Binti Wan Azlan<br />

Supervisor: Dr. Zamzahaila Binti Mohd Zin<br />

Bachelor of Food Science (Food Service and Nutrition)<br />

School of Food Science and Technology<br />

Piper sarmentosum is an herbal plant. It is commonly used in medicinal herbs as<br />

treatment of toothache, dermatitis, and asthma. It is a good source of natural antioxidant.<br />

In this study, various concentrations (50%, 75% and 100%) of methanol and ethanol will<br />

be used as solvent extraction of Piper sarmentosum. The antioxidant activity, total<br />

phenolic content, total flavonoid content of the freeze-dried Piper sarmentosum extracts<br />

will be investigated. Antioxidant activity will be determine by free radical scavenging<br />

activity (DPPH) and Ferric Reducing Antioxidant Power (FRAP) method while Total<br />

Phenolic content (TPC) will be determine by using Folin-Ciocalteu (FC) assay and<br />

determination of Total Flavonoid Content (TFC) using aluminum chloride colorimetric<br />

assay. Piper sarmentosum will be a good source of antioxidant, total phenolic content<br />

and total flavonoid content that can be apply in medicine purpose and can reduce<br />

oxidative stress in dietary application.<br />

1215 | UMT UNDERGRADUATE RESEARCH DAY

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