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Data Mining and Phylogenetic Analysis of Pangasius nasutus from based
on 16s rRNA gene sequences
Rabiatul Dzurifah bt Ali
Supervisor: Dr Nurasma bt Ariffin
Bachelor of Science Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Pangasius nasutus or referred as Pangasiid catfish locally known as ‘Patin Buah’ is river
catfish natively endemic in Pahang River in Malaysia. It is listed as one of the species
threatened to extinction based on the IUCN Red List where numbers of catch of this
fish have been declining. A study was conducted to determine the phylogenetic
relationships of Pangasius nasutus based on 16s rRNA gene sequences. 20 sequences
were collected from previous study and 6 sequences were downloaded from The
National Center for Biotechnology Information. Phylogenetic trees were constructed
using Maximum Parsimony, Maximum Likelihood and Neighbor-joining method on
MEGA7 software. The results shows the first group comprise haplotype among the 20
samples of Pangasiid Catfish Maran and Temerloh while the second group include
haplotype from Thailand. Current data can be used as data sources for the future to
increase the production of the Pangasius nasutus through breeding as well.
529 | UMT UNDERGRADUATE RESEARCH DAY 2018