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Molecular Detection of Megalocytivirus on Outer Tissue of Marphysa
Moribidii Collected from Morib, Selangor
Siti Zaima Binti Zainal
Supervisor: Dr. Siti Nor Khadijah Binti Addis
Bachelor of Science (Biological Science)
School of Fundamental Science
Previous study has shown that polychaete, Marphysa sp. has become a passive carrier
for White Spot Syndrome Virus (WSSV) whereas Megalocytivirus can co-exist with
WSSV. On account to this matter, there is a possibility of Megalocytivirus also possible
to be detected in polychaete. This study elaborated the first detection of
Megalocytivirus in new organism; Marphysa moribidii. Five extracted samples collected
from Morib, Selangor were analyzed by using nested polymerase chain reaction (PCR)
with primers that are selectively amplified major capsid protein (MCP) of extracted
DNA samples. The PCR products were screened to determine the expected size which
is 430 bp (for primary PCR) and 167 bp (for secondary PCR). Overall, the results shown
two out of five samples used in this study were found to be Megalocytivirus positive,
indicating the presence of Megalocytivirus on the outer tissue of M. moribidii collected
from Morib. This preliminary study shown that Megalocytivirus can also be detected
on M. moribidii collected from Morib, Selangor.
339 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018