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Population Structure Revealed by Mitochondrial Control Region and 16S<br />

rRNA of Penaeus Monodon (Fabricius, 1798) Broodstocks in the Indo-<br />

Pacific Region<br />

Amir Asyraf Bin Zainudin<br />

Supervisor: Dr. Li Lian Wong<br />

Bachelor of Applied Science (Fisheries)<br />

School of Fisheries and Aquaculture Sciences<br />

The Asian tiger shrimp, Penaeus monodon (Fabricius, 1798) is the most economically<br />

important group of shrimps, which is widely cultured in the world. Genetic variation is<br />

a critical factor when selecting superior stocks for aquaculture breeding programs.<br />

Current study focuses on determining the genetic diversity, population structure and<br />

phylogeographic relationships of different Penaeus monodon populations from the<br />

Indo-Pacific region. Based on the concatenated sequences between 16s rRNA and<br />

mitochondrial control region gene, 12 haplotypes are recognised from 73 samples<br />

collected from Malaysia (n=7), Madagascar (n=28), Thailand (n=18), Hawaii (n=11)<br />

and Japan (n=9). Analysis showed low/high haplotypes diversity (0.766) and<br />

nucleotide diversity (0.25490). The Fst scores (-0.03251 to 0.74404) indicated high<br />

genetic structure of P. monodon populations.<br />

668 | UMT UNDERGRADUATE RESEARCH DAY 2018

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