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ENZYMATIC POTENTIAL OF BACTERIA ISOLATED FROM GREEN SEA<br />

TURTLE (Chelonia mydas)<br />

Farah Fazida Binti Md Ishak<br />

Supervisor: Dr. Jasnizat Bin Saidin<br />

Bachelor of Science (Marine Biology)<br />

School Of Marine and Environment Sciences<br />

Universiti Malaysia Terengganu<br />

Global market of industrial enzymes estimated around US$4.2 billion in 2014<br />

and expected to reach nearly US$6.2 billion in 2015-2020. The vital sources of<br />

industrial enzymes are from microorganisms especially bacteria. Researcher are<br />

searching every possible environmental niche for potential enzyme-producing<br />

bacteria which include animal-associated. This study investigated the potential<br />

enzyme-producing bacteria from endangered animal which is green sea turtle<br />

(Chelonia mydas). Six bacteria have been isolated from cloaca mucus of sea<br />

turtle. All of these isolates positively produce lipase when supplemented with<br />

olive and corn oil while only three bacteria were able to utilize used-cookingoil.<br />

Oppositely, none of the isolate shows amylase activity when starch used as<br />

substrate. As for protease enzyme, three bacteria showed production.<br />

Unidentified bacteria strain T6M was able to produced lipase with all oil<br />

substrate used and produced protease. This strain is a potential candidate for<br />

further investigation on it enzymes characteristic.<br />

1793 | UMT UNDERGRADUATE RESEARCH DAY 2018

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