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Data Mining and Phylogenetic Analysis of Pangasius Nasutus Based On<br />

Cythochrome C Oxidase 1 (CO1) DNA Sequences<br />

Nur Izzah Binti Mohamad Yadzir<br />

Supervisor: Dr Nur Asma Binti Ariffin<br />

Bachelor of Sciences in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

Pangasiidae is known as a large family of tropical freshwater catfishes that can be<br />

found throughout South and Southeast Asia. Catfish species of this family has a good<br />

potential for aquaculture and high values as flesh food at market in Malaysia. The<br />

molecular phylogenetic trees of 20 representative sequences of Pangasius nasutus<br />

using cytochrome c oxidase subunit I (COI) were constructed. The objective of this<br />

study is to study the phylogenetic analysis of Pangasius nasutus based on COI DNA<br />

sequences. The sequences COI available in National Centre Biology Information<br />

(NCBI) for Pangasius nasutus have been compiled. Then the sequences were aligned<br />

and tree were constucted in Mega software. The results clearly showed that COI gene<br />

can be used to give a clear picture of the phylogenetic relationships among different<br />

species of family Pangasiidae. The method developed in the present study is less timeconsuming<br />

and easy to execute. The technique also provide valuable information for<br />

breeding and conservation programme.<br />

566 | UMT UNDERGRADUATE RESEARCH DAY 2018

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