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Isolation and Identification of Rhamnolipid Producing Bacteria from<br />

Mangrove Environment<br />

Nurul Nasuha binti Zaidi Kobli<br />

Supervisor: Assoc. Prof. Dr. Kesaven Bhubalan<br />

Bachelor of Applied Science<br />

(Biodiversity Conservation and Management)<br />

School of Marine and Environmental Sciences<br />

Universiti Malaysia Terengganu<br />

Surfactant have been used in our daily life such as detergent, cosmetic products<br />

and in industrial. Rhamnolipid is one of the biosurfactant which can be produced<br />

by microorganisms, have advantageous characteristic such as low toxicity, high<br />

biodegradability, environmental friendly compared to surfactant. It can be<br />

produced by various types of carbon source with a low production cost such as<br />

palm oil, glycerol. This aim of this study was to identify the rhamnolipid<br />

producing bacteria from mangrove environment and to determine the amount<br />

of rhamnolipid produce and its surface activity. Seven types of bacteria was<br />

isolated from mangrove water and palm oil and glycerol have been used as a<br />

carbon source. The screening techniques employed were drop collapse method<br />

and emulsification index. Based on emulsification index, only six of bacteria that<br />

capable to produce the rhamnolipid biosurfactant.The bacteria were identified<br />

and this study showed that mangrove have some bacteria that capable produce<br />

rhamnolipid.<br />

1755 | UMT UNDERGRADUATE RESEARCH DAY 2018

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