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Assessment of Free R-Glycidol Compound Cytotoxicity Mechanism in
Human Colon Cell Line, Hct 116 via Mapk Pathway
Siti Nur Syahirah binti Nor Mahiran
Supervisor: Dr Nurul Huda binti Abdul Kadir
Bachelor of Science (Biological Sciences)
School Of Fundamental Science
Glycidol is one of contaminants that arise in food especially in refined cooking oils and
toxic due to its electrophilic properties that lead to colon cancer. Cytotoxicity effect of
glycidol in human cells was carried out to ensure understanding on the mechanism.
Free R-glycidol compound possess cytotoxic effect on HCT 116 cells were evaluated
using AlamarBlue ® assay. It showed significant potential to reduce cell viability with
exclusive 50% inhibition concentration (IC50) each time point of treatments (IC50:
5.01, 4.47 and 3.41 mM after 24, 48 and 72 hours). Reactive oxygen species activity
was corresponded with morphological changes of the cells after stained with
dichlorofluorescin diacetate (DCFDA) showed apoptosis mode of cell death. ERK ½,
p-ERK, BCL-2 and Caspase-3 mitogen-activated protein kinases was detected using
western blot technique. The result shown protein expression was down regulated after
48 hours, suggesting cell death event induced by free R-glycidol compound due to
apoptosis
391 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018