merged

Data mining and phylogenetic analysis on cytochrome b mitochondrial
gene of seahorse (Hippocampus kuda)
Nurul Liana Binti Azeman
Supervisor: Dr Nur Asma Binti Ariffin
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Hippocampus kuda have been listed as an endangered species due to recent record
shows declining in all over the world. It may due to target catch , incident capture and
habitat degradation. Genetic distance of Hippocampus kuda on cytochrome b were
being determine to choose the best for conservation. There are 26 sequences being
downloaded from GenBank and compile in a file. The sequences undergoes alignment
and used for construct phylogenetic tree. For Maximum Likelihood tree , there are two
distinct group of Hippocampus kuda samples.For Neighbor-Joining tree of cytochrome
b shows all sequences are in one lineage. Maximum parsimony tree, the consistency
index is 0.9184894 , retention index is 0.828571 and composite index is 0.758055.
This result shows genetic distance between the Hippocampus kuda . Further study on
breeding of the best Hippocampus kuda should be done to prove and strengthen this
experiment data.
525 | UMT UNDERGRADUATE RESEARCH DAY 2018