merged

Molecular Detection of Megalocytivirus in Diopatra Claparedii through
Endodermis
Muhammad Nor Ishraff bin Adnan
Supervisor: Dr. Siti Nor Khadijah binti Addis
Co. supervisor: Dr. Sandra Catherine Zainathan
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Megalocytivirus have been causing mass mortalities in various marine and freshwater
fishes of Asian countries. Thus, the purpose of the study is to to investigate the
presence of Megalocytivirus in endoderm of Diopatra claparedii by polymerase chain
reaction. This research will be conducted to determine the existence of Megalocytivirus
in Diopatra claparedii where polychaetes is known to be the viral vector for White Spot
Syndrome Virus (WSSV). Diopatra claparedii was collected in Morib, Selangor. Total
genomic DNA of 5 sample of Diopatra claparedii was extracted. The result was
obtained by carrying Polymerase Chain Reaction (PCR). This study involved the use of
2 positive control (extracted DNA from infected oyster and plasmid contain MCP gene
of Megalocytivirus), and a negative control (with no DNA template) as to validate the
laboratory findings. At the end of the study, the Megalocytivirus cannot be detected
where the bands cannot be observed through agarose gel electrophoresis due to
incorrect method and contaminated of samples.
338 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018