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Effect of Larval Food Amount on In-vitro Rearing of Queen Stingless<br />

Bee, Heterotrigona Itama (Hymenoptera: Apidae: Meliponini)<br />

Devadarsheene A/P Regena<br />

Supervisor: Prof. Madya Dr Wahizatul Afzan Azmi<br />

Co-supervisor: Prof. Madya Dr Shamsul Bahri Abd. Razak<br />

Bachelor of Applied Science<br />

(Biodiversity Conservation and Management)<br />

School of Marine and Environmental Sciences<br />

The in-vitro rearing of queen stingless bee is one of the efforts to increase the<br />

colonies’ number in order to produce more stingless bee products such as<br />

honey, propolis and bee bread. To date, there are limited studies on the<br />

production of the stingless bee queens by manipulating the quantity of food to<br />

the larvae. Thus, the aim of this study were to determine the growth rate of<br />

the in-vitro rearing of queen stingless bee and to investigate the optimum<br />

amount of larval food needed by larvae. Three different treatments of amount<br />

of larval food were tested on the larvae and the measurement of head capsule<br />

width was taken every week until the emergence stage. Results showed that<br />

the diameter of head capsule of larvae increased from week 1 to week 11 but<br />

there was no queen produced due to contamination of the treatment. Survival<br />

rate was higher in 100µl of larval food compared to 120µl and 140µl. Further<br />

studies is needed to improve the technique of the in-vitro rearing.<br />

1687 | UMT UNDERGRADUATE RESEARCH DAY 2018

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