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Screening and Identification of Enzyme Producing Gram-positive Bacteria
Isolated From Mangrove Soil in Setiu Wetlands, Terengganu
Aina Nabila binti Abdul Rahman
Supervisor: Dr. Jasnizat bin Saidin
Bachelor of Science (Marine Biology)
School of Marine and Environmental Sciences
Demands for industrial enzymes are continuously increasing due to economic
development in term of products manufacturing. The most important source of
producing these industrial enzymes are bacteria especially Gram-positive bacteria.
Gram-positive bacteria are well-known as the producers of stable and high capacity
industrial enzymes due to their vigorousness, thermostability and bulk yield. This study
explored mangrove ecosystems as a resource of extracting Gram-positive bacteria.
Fifteen Gram-positive bacteria were isolated from mangrove soil and tested for
amylase, lipase and protease production. Eleven isolates exhibited at least one of the
enzyme activity with different intensity of clearing zone around the colony. Three of
the isolate identified as Bacillus velezensis, B. cereus and B. nakamurai were capable
of producing all of the three enzyme tested. These bacteria species are potential
candidates for further analysis by enzymatic assay to maximize enzyme activity and
stimulate in larger scale of production for biotechnological and industrial applications.
1812 | UMT UNDERGRADUATE RESEARCH DAY 2018