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Enhancement of Exopolysaccharides (Eps) Production with Addition of
Water-Soluble Polysaccharide Fermented By Bacillus Cereus from
Polychaete Guts
Mohamad Ikmal Naim Bin Mutaza
Supervisor: Dr. Azila binti Adnan
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Polychaete also known as brittle worm are diverse class worms. For this project,
fermentation process has been used to produce EPS by Bacillus cereu species. EPS
are essential to increase the viscosity and improve the texture of fermented milks
however the magnitude varies depending on incubation condition of total solid
content, viscosity measurement and culture strain. Hence, this study elucidated the
effect of water-soluble polysaccharides on EPS yield produce by polychaete bacteria.
Fermentation went for 24 hours and under controlled temperature, shaker rotation
and pH value. Bacterial growth, sugar consumption and productivity of EPS were
compared between three types of culture media. Nutrient agar shows the highest
compared to xanthan and non-water-soluble polysaccharides media. It is appeared
that media influences the increasing number of bacteria activity, thus effecting the
sugar consumption and EPS production.
358 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018