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Total Phenolic and Flavonoid Concentration of Eco Enzyme from
Sugarcane Bagasse and Their Potential Applications in Red Palm Weevil
(Rpw) Integrated Pest Management Approach
Aina Mardia binti Borhan
Supervisor: Dr. Hazlina binti Ahamad Zakeri
Co-supervisor: Assoc. Prof. Dr. Wahizatul Afzan Azmi
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Red palm weevil (RPW), Rhynchoporus ferrugineus, is a most major pest of palm trees
which are the most important source of economy worldwide. It causing problems
toward farmers and utilising eco-enzyme has been introduced to overcome the
infestation. Purpose of this study were to determine the total phenolic concentration
(TPC) and total flavonoid concentration (TFC) of sugarcane eco-enzyme that could be
give effect to cellulase activity inhibition and anti-feedant activity of RPW. The TPC
and TFC were determined using Follin-Ciocalteau method and Dowd method
respectively. Cellulase activity inhibition was determined by using carboxymethyl
cellulose agar as a substrate while using flour disk assay to determine anti-feedant
based on feeding deterrent index (FDI). The results obtained showed that sugarcane
bagasse eco-enzyme was inhibitor for cellulase activity and stimulate the feeding of
RPW. In conclusion, sugarcane bagasse eco-enzyme has a potential to manage RPW
and helps in reducing waste production.
325 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018