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Prevalence of Foodborne Bacteria and Their Occurrence Of Antibiotic
Resistance Bacteria And Heavy Metals In Tilapia Sold In Wet Markets, Kuala
Terengganu.
Safiyyah Binti Ismail
Supervisor: Dr. Mohd Nizam Bin Lani
Co-Supervisor: Dr. Ong Meng Chuan
Bachelor of Food Science (Food Technology)
School of Food Science and Technology
Tilapia or Oreochromis mossambicus is a hardy, fast-growing fish in shallow water that
has been an important source of protein in many developing countries, including Malaysia.
It has become the second most commonly consumed farmed fish after carps.
Environmental conditions and feeding habit may expose the fish to microbial and chemical
hazards. Therefore, this study will investigate the prevalence bacteria (Vibrio, E. coli, S.
aureus, Pseudomonas and Aeromonas) associated with Tilapia and determine the
occurrence of antibiotic resistance bacteria and heavy metals in Tilapia from several wet
markets in Kuala Terengganu. The bacteria was obtained from fish flesh, inner organ and
gills will be isolated using selective media and identified using API 20E and 20NE Kit. The
antibiotic susceptibility (oxytetracycline, tetracycline, florfenicol, sulfadimethoxine,
ormetoprim and virginiamycin) of the identified bacteria will be analyzed using disk
diffusion method. Then, four types of heavy metals (Cadmium, Copper, Lead and Zinc)
will be measured using atomic absorption spectrophotometer and mercury will be
measured with Mercury analyzer. Results obtained will determine the status of microbial
and chemical hazards of Tilapia sold in wet markets in Kuala Terengganu.
1292 | UMT UNDERGRADUATE RESEARCH DAY