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Isolation and Identification of Acanthamoeba Healyi at Diopatra
Claparedii
Siti Erma Rusniza bt Abd. Rashid
Supervisor: Dr. Fatimah Hashim
Co-supervisor: Dr. Malinna Binti Jusoh
Bachelor of Science (Biological Sciences)
School of Fundamental Science
In 2017, 100% presence of amoeba were observed in cyst and trophozoite form that
cultured from Dioptara claparedii which sampled in Morib beach, Selangor by using
swabbing technique. The presence of cyst and trophozoite were confirmed by using
light microscope and scanning electron microscope (SEM) where it revealed the
average diameter size of 30 amoeba cysts (16.14µm ± 1.63) and the trophozoites’s
average length (23.46µm ± 5.59). SEM observation showed the presence of ostioles
on the surface of the cyst. This study was undergo Polymerase Chain Reaction (PCR)
to identify the species of the amoeba. The result obtained from the PCR shows 98%
similarity to Acanthamoeba healyi under accession number of AF019070.1 with 94%
query cover and 731 maximum score. The presence of A. healyi fulfilled the objective
of this study that is to identify the species of amoeba presence in D. claparedii and it
allow the roles for the polychaete to be studied more in the future.
337 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018