merged

Isolation of probiotics bacteria from Asian redtail catfish
(Mystus nemurus) gut
Hanis syuhaidah binti Mohamed Ashri
Supervisor: Dr Shumpei Iehata
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Mystus nemurus is one of the most highly demand fish in Southeast Asia, but the
growth was slow because of fungal and bacterial infections such as Aeromonas sp.
Probiotics usage is the alternative ways to control the various infections toward the
fishes with minimizing the uses of antibiotic substances.The aim of this study was to
isolate possible probiotics bacteria candidates from M.nemurus gut based on
antipathogenic activity. Probiotics bacteria candidates were isolated by using pour
method onto MRS agar with 1.5% CaCO3 and incubated at room temperature for 3
days.The isolates were determined whether they had growth inhibition of pathogen
Aeromonas sp. using the well diffusion method and double layer agar method. Positive
isolate was further identified by polymerase chain reaction (PCR) and sequencing.
Initially, 9 isolates were found from M.nemurus gut. One of nine isolate showed
suppression of Aeromonas hydrophila growth. The sequencing are now in progress.
487 | UMT UNDERGRADUATE RESEARCH DAY 2018