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In Vitro Examination on Tetrahydrozoline Hydrochloride Efficacy of On
Acanthameoba Sp. A Causative Agent for Acanthamoeba Keratitis.
Zafirah Najwa Binti Zainal Abidin
Supervisor: Dr. Fatimah Hashim
Co-Supervisor: Dr Muhammad Fairus Bin Noor Hashim
Bachelor of Science (Biological Sciences)
School of Fundamental Science
The risen of Acanthamoeba keratitis (AK) cases in the recent years proven the current
treatments of the AK still not fully effectives. In this study the Acanthamoeba sp. was
exposed to the different concentration of tetrahydrozoline hydrochloride (THCl) to
study the efficacy of the compounds towards Acanthamoeba sp. In this study, the IC50
concentration obtained from MTT assay of the THClwas 45.1%. Morphological
changes of Acanthamoeba cells after has been treated with the IC50 concentration for
24 hours was observed under the light microscope showed the Acanthamoeba cells
rounded in shape indicating the encystment process from its trophozoite stage. From
the observations after performed the treated Acanthamoeba cells with AO/PI staining,
the green cytoplasm stained red which indicated the non-viable cells while the orange
granules in cytoplasm indicated the necrosis of cell death. This study found that
THClthat have been marketed and used for eye infection treatment is not really
effective on Acanthamoeba sp. in this study proved.
Keywords: Acanthameobasp., Acanthamoeba keratitis,cytotoxicity,tetrahydrozoline
chloride
334 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018