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Breeding response and larval quality of the hybrid between Clarias<br />

gariepinus and Pangasianodon hypophthalmus using different dosage<br />

of ovaprim<br />

Mohamad Izkram bin Ibrahim<br />

Supervisor: Dr Siti Ariza binti Aripin<br />

Bachelor of Science in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

This study was designed to evaluate the trait performance using different dosage of<br />

hormones ovaprim. Hormones ovaprim had been used to induce the final oocyte<br />

maturation and spawning in fish. Hybrids between Clarias gariepinus and<br />

Pangasianodon hypophthalmus were obtained by hormone injection of brood stock<br />

and artificial injection. Three different dosages (0.3 ml/kg, 0.4 ml/kg and 0.5 ml/kg)<br />

had been used and the concentration of the sperm of P. hypophthalmus is ranging<br />

from (18.4× 10 5 -21.3× 10 5 /ml. In this study, for fertilization rate, hybrid<br />

♀Pangasianodon hypophtalamus ×♂Clarias gariepinus (Pangapinus) is not significantly<br />

difference and ranging from 42%-52% (p:0.057) . Hatching rate is significantly high<br />

which is from 5.5%-13.9% (p:0.002). Survival rate is not significantly difference and<br />

ranging from 22.22%-77.51% (p:0.079). Finally, abnormal rate is not significantly<br />

difference and ranging from 33.33% – 83.01% (p:0.450).). Present result showed that<br />

treatment 3 with 0.5 ml/kg ovaprim is preferable for the crossbreeding of<br />

Pangasianodon hypophthalmus and Clarias gariepinus.<br />

493 | UMT UNDERGRADUATE RESEARCH DAY 2018

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