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Isolation And Identification Of P[3HB-CO-4HB] Producing Bacterial Strain
From Various Environment In Kuala Terengganu
Manogiri A/P Sadasivam
Supervisor: Dr.Vigneswari Sevakumaran
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Polyhydroxyalkanoates (PHA) are the microbiologically synthesized plastic. Among all
types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) is widely
sought after. The aim of this study is to isolate and identify P(3HB-co-4HB) producers
from various environment in Kuala Terengganu. Hence, samples of soil and water
were collected and screened for P(3HB-co-4HB) producer. A total of 18 isolates were
isolated, however only 5 isolates were identified as potential PHA producer.
Interestingly, 3 isolates were confirmed as copolymer P(3HB-co-4HB) producer
through gas chromatography analysis. These 3 isolated strains were then identified
using BBL Crystal Kit. It was identified as Burkholderia sp., TMT 11 (0.975 of
confidence value) with highest 4HB molar fraction of 14.1%. The other 2 isolates are
Shigella sp., KPD 13 (0.8728 confidence value) and Acinetobacter sp., PD 16 (0.2969
confidence value). The properties of the copolymer P(3HB-co-4HB) produced by the
wide variety of bacteria isolated can be tailored for various biomedical applications.
Keywords: Polyhydroxyalkanoates, poly(3-hydroxybutyrate-co-4-hydroxybutyrate),
Burkholderia sp., Shigella sp., Acinetobacter sp.
399 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018