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Identification of Pangasiid Catfish (Pangasius Nasutus) from Maran and
Temerloh using PCR-RFLP on Cytochrome B Gene
Wan Fazlina binti Wan Zahari
Supervisor: Dr. Nur Asma binti Ariffin
Bachelor of Applied Science (Fisheries)
School of Fisheries and Aquaculture Sciences
Morphologically, family Pangasiidae is recognized by a laterally compressed body,
having two pairs of barbels, a short dorsal fin with two spines, a developed adipose
fin, a long anal fin, and a strong pectoral spine (Gustiano, 2009). Catfishes are known
to occupy habitats ranging from upload streams to large river channels and to seasonal
floodplain lagoons (Winemiller and Kelso-Winemiller, 1996). Therefore, it is not a
surprise that P. nasutus was known to occur in the Pahang River where the richness
of the biodiversity is at its best among the rivers in Malaysia. (Haslawati et al, 2004).
DNA barcoding is a useful technique for the molecular identification of organisms.
Species identification of Pangasiid catfish or known as Pangasius sp. using PCR-RFLP
method on cytochrome b gene. In this study, the determination prospective molecular
markers for detecting Pangasius species including Pangasius nasutus had been done.
Some of the sequences were taken from NCBI website for Pangasius species. The
restriction enzyme had been used to digest the sequences. PCR-RFLP markers have
been used with success in detecting frauds in the fishery trade (Espiñeira et al., 2009).
The PCR- Restriction Fragment Length Polymorphism had been used in this study to
distinguish between Pangasius nasutus species and Pangasius nasutus with other
Pangasius species. Data analysis was done by using Restriction Mapper software. The
suitable marker for differentiating Pangasius nasutus from other Pangasius species
consist of cytochrome b gene and digestion by NcoⅠ and StyⅠ restriction enzyme.
The uses of RFLP method is very simple as it is inexpensive and less time consuming
and also, one of the promising technique.
653 | UMT UNDERGRADUATE RESEARCH DAY 2018