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Comparison of Two Housekeeping Genes (18s Rrna and Gapdh) of
Microalgae (Chlorella Vulgaris) Grown Under
Nitrogen Limited Condition
Nor ‘Awatif binti Che Soh
Supervisor: Dr. Malinna binti Jusoh
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Real-time polymerase chain reaction (PCR) is crucial in gene expression analysis and
it necessitates housekeeping genes (HKGs) as internal standard controls. It is essential
to establish stable reference genes for the proper normalization. This study aimed at
evaluating the stability of two HKGs (18S rRNA and GAPDH) of Chlorella vulgaris grown
under nitrogen limited condition. C. vulgaris were grown in complete F/2 media with
100% and 25% of NaNO3 concentration for control and treatment condition,
respectively. The cultures were grown for 28 days and RNA extraction was done for
every 7 day intervals. Cycle threshold (Ct) values generated from real-time PCR
showed a significant stable expression of GAPDH in control and treatment conditions
which were 37.23 ± 0.30 and 36.44 ± 0.53, respectively as compared to 18S. Thus,
GAPDH can be used as a reference gene in the study of gene expression of C. vulgaris
for lipid production.
351 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018