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Investigation of Free S-Glycidol Compound Cytotoxicity Mechanism in
Human Colon Cell Line (Hct 116) Via Mapk Pathway
Nanthini Ravi
Supervisor: Dr. Nurul Huda Binti Abdul Kadir @Abdul Rahman
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Human Colorectal Cancer is classified as the third most common cancer in both men
and women although the risk is slightly lower in woman. Free S Glycidol Compound
cytotoxicity was investigated against Human Colon Cell Lines (HCT-116). The cytotoxic
effect of HCT-116 cells were evaluated by using AlamarBlue ® assay. The compound
showed significant potential to reduce the viability of the HCT-116 cells with exclusive
50% inhibition concentration (IC50) on each time of the treatments (IC50: 4.30mM
after 24 hours; 0.656mM after 48 hours; 0.398mM after 72 hours). GAPDH, ERK1/2,
p-ERK, BCL-2, CASPACE-3 mitogen-activated protein kinase (MAPK) was detected
using Western blot technique and our results had shown that the protein expression
of treated HCT-116 with S Glycidol compound for CASPACE-3 was down-regulated for
both 24 and 48 hours indicating no apoptosis involved although BCL-2 shows preapoptotic
expression at 24 hours, suggesting that cell death event was initially
triggered but underwent homeostasis. In line, with the result of protein expression of
CASPACE-3, there is no induction of apoptosis via ROS generation after treated for 24
hours, thus suggesting that the cell death event does not occur after the treatment
with Free S Glycidol Compound.
392 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018