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Detection of Dengue Virus in Aedes Sp. Eggs in Seberang Takir,
Terengganu
Dianah Irdina Binti Mohd Ibrahim
Supervisor: Dr. Suzana Misbah
Bachelor of Science (Biological Sciences)
School of Fundamental Science
This study was conducted to detect the presence of dengue virus (DENV) in Aedes sp.
eggs of Seberang Takir, an area with high dengue cases reported. Eggs were collected
using standardized ovitraps and were subjected to RNA isolation. A total of 1,608 eggs
were collected within October 2017 to January 2018. Transovarial transmission of
DENV in mosquitoes was then determined using reverse transcriptase-polymerase
reaction (RT-PCR). From 28 pools of egg screening, there was no sample found to be
positive for DENV. This gives a minimum infection rate (MIR) of zero for the eggs
carrying DENV. Data from this study conclude that there is a low risk of DENV
transmission within Seberang Takir. Further virological surveillance is necessary to
predict and control dengue outbreak in the future.
345 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018