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Isolation and Identification of Hydrocarbon-Degrading Bacteria Isolated
From Mud Soil
Nur Maisarah Binti Mohd Jaafar
Supervisor: Dr. Fazilah Binti Ariffin
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Polycyclic aromatic hydrocarbons (PAHs) are becoming worldwide problem
environmental pollutants. Presence and disposal of PAHs is highly concerned due to
its toxicological effects to the soil ecosystems. This study was conducted to isolate
and identify the hydrocarbon-degrading bacteria isolated from mud soil that were
collected from Pantai Kelanang Morib, Selangor and to study the diesel oil degradation
ability of bacteria. The significance of this study determined bacteria with their high
ability to degrade hydrocarbons can be mass cultured in the future to remove toxic.
The degradation analysis was carried out for 20 days of incubation in rotary shaker at
37˚C, 150 rpm. Two hydrocarbon-degrading bacteria were identified based on BBL
Crystal Identification Kit as Bacillus cereus sp. shown 97.34% and Corynebacterium
bovis sp. shown 98.96% of diesel oil degradation in 20 days. The bacterial
biochemistry during bioremediation of PAH should be widely studied so that it will be
beneficial for bioremediation of hydrocarbon-contaminated soil.
371 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018