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Pre-screening of Antibacterial and Antioxidant of Methanolic Crude Extract
of Local Cultured Red Seaweed Gracilaria sp.
Nur Adilah Binti Mat Azmi
Supervisor: Dr. Suvik Assaw
Bachelor of Science (Marine Biology)
School Of Marine and Environmental Science
Seaweeds have been proven to possess pharmaceutical and therapeutic values, and
also rich sources of bioactive compounds which can be developed to fight against
bacterial infections and cells oxidation. The purpose of this study is to determine
antibacterial and antioxidant of methanolic crude extract of local cultured Gracilaria
sp. The crude extract was tested for antibacterial activity against Bacillus subtilis,
Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Vibrio cholera
and Enterobacter cloacae using well diffusion method. Tetracycline was used as
positive control. Antioxidant activity of the crude extract is analysed through DPPH
free radical scavenging assay and was compared with standard Quercetin. Results
revealed that the methanolic crude extract possessed weak antibacterial properties
and only inhibited B. subtilis, S. aureus, E. coli and V. cholera. Minimum Inhibitory
Concentration (MIC) evaluated to be from 0.156-1.25 mg/mL. The crude extract
showed moderate antioxidant activity (IC50=5.6 mg/mL). Present findings suggest that
Gracilaria sp. could be a potential natural source of antioxidant.
1827 | UMT UNDERGRADUATE RESEARCH DAY 2018