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Rate of Decomposition of Abdomen Fatty Flesh of a Cadaver Buried in<br />

Nami Series Soil of Bukit Khor<br />

Chong Zan Yang<br />

Supervisor: Dr. Siti Sofo binti Ismail<br />

Bachelor of Science (Analytical and Environmental Chemistry)<br />

School of Marine and Environmental Sciences<br />

Universiti Malaysia Terengganu<br />

The identification of a buried cadaver become challenging when the cadaver is badly<br />

decomposed. Therefore, lipid can act as the bio-indicator to determine the<br />

anthropogenic signal from the clandestine grave, subsequently, to determine the<br />

postmortem interval. For this study, the rate of decomposition, pH, total organic<br />

carbon (TOC), total lipid extracted (TLE), lipid concentration and phospholipid fatty<br />

acids (PLFAs) was determined in each decomposition stages. A control laboratory<br />

experiment was carried out to mimic a burial in shallow grave under tropical climate.<br />

Modified Bligh Dyer method was utilized to extract the lipid from the soil. The lipid<br />

was fractioned to obtain the PLFAs. GC-FID and GC-MS was used to determine the<br />

concentration of lipid and types of PLFAs respectively. It is found that different<br />

decomposition stage show different result of the rate of decomposition, pH, TOC, TLE,<br />

concentration of the lipid and types of the PLFAs. Therefore, the soil lipids is a potential<br />

forensic tool in located a clandestine grave, identify of the burial cadaver as well as<br />

increase the accuracy to estimate the PMI.<br />

1939 | UMT UNDERGRADUATE RESEARCH DAY 2018

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