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Optimization of Micro-Solid Phase Extraction Method for Caffeine Analysis
in Water Using Central Composite Design
Nurul Aryani Binti Zulkipli
Supervisor: Dr. Wan Mohd Afiq Bin Wan Mohd Khalik
Co-Supervisor: Dr. Loh Saw Hong
Bachelor of Science (Analytical and Environmental Chemistry)
School of Marine and Environmental Sciences
Universiti Malaysia Terengganu
Caffeine widely consumed in food, beverages, medicine and pharmaceutical products.
There are many ways for caffeine to introduce into the aquatic environment including
disposal of effluent from domestic wastewater treatment plant or industrial waste. The
research work aims to develop the optimum condition for extraction of caffeine residue
in water. Micro-solid phase extraction (μ-SPE) method has been developed using C18-
impregnated cellulose triacetate as an adsorbent film. The optimum condition
suggested by central composite design model as follow; a number of the film (1 piece),
pH water sample (9), stirring rate (200 rpm), and extraction time (30 minutes). Under
optimum condition, the developed method showed excellent linearity (r 2 = 0.999),
good recovery (92.6-94.8%) and low detection limit (0.13 ng/ml). The developed μ-
SPE method is considered simple, environment-friendly and inexpensive. Caffeine
residue in freshwater sample was found at low levels concentration, 6.98 and 18.23
ng/ml when extracted using μ-SPE method.
1963 | UMT UNDERGRADUATE RESEARCH DAY 2018