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Electrochemical Biosensor for Domoic Acid<br />

Lai Yun Ru<br />

Supervisor: Dr. Hafiza binti Mohamed Zuki<br />

Co-Supervisor: Dr. Azrilawani Ahmad @ Othman<br />

Bachelor of Science (Analytical and Environmental Chemistry)<br />

School of Marine and Environmental Science<br />

Universiti Malaysia Terengganu<br />

An electrochemical biosensor was successfully developed using modified screenprinted<br />

carbon electrode (SPCE). All electrode measurements were conducted using<br />

cyclic voltammetry (CV) in the presence of 5 mM ferricyanide in 0.1 KCl electrolyte<br />

with the scan rate of 50 mV/s at a potential from -0.2 V to 0.6 V. Indirect competitive<br />

enzyme-linked immunosorbent assay (ELISA) had been applied for the immobilisation<br />

of biomolecules on SPCE. Influences of coating buffer and blocking buffer were studied<br />

and optimized. Concentration of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic<br />

acid receptor (AMPA-R), domoic acid (DA) and goat anti-mouse alkaline phosphatase<br />

(IgG-AP) were 1.3 ppm, 0.8 ppm and 1.7 ppm respectively. The LOD calculated for<br />

the electrochemical biosensor was 1.38 ppm. The low value of LOD proves the high<br />

sensitivity of the electrochemical biosensor developed.<br />

1945 | UMT UNDERGRADUATE RESEARCH DAY 2018

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