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PRODUCTION AND PURIFICATION OF POLYSACCHARIDE PRODUCE FROM<br />

BACTERIA (Brevibacillus borstelensis) ASSOCIATED WITH HORSESHOE<br />

CRAB (Tachypleus gigas)<br />

Nursakinah binti Ngaspan<br />

Prof. Madya Dr. Ahmad Shamsuddin bin Ahmad<br />

Bachelor of Sciences (Marine Biology)<br />

School of Marine and Environmental Sciences<br />

Bacteria are often labelled as bad organisms as it causes of human and animal<br />

diseases. However, despite these characteristics, there are certain bacteria can bring<br />

good benefits to us such as the bacteria producing polysaccharides. Previous reports<br />

indicating the production of secondary metabolites by marine bacteria have yielded<br />

medical products such as novel anti-inflammatory, anti-cancer and antibiotic agent.<br />

The main aim of this study was to determine the production of polysaccharide in term<br />

of their sugar composition from the selected isolates of bacteria associated with<br />

horseshoe crab, Tachypleus gigas. The bacteria were identified as Brevibacillus<br />

borstelensis by using vitek 2 systems. The results showed that there are three type of<br />

sugars isolated; maltose, glucose and rhamnose in the polysaccharide produced by<br />

using paper chromatography (PC) and High-Performance Liquid Chromatography<br />

(HPLC). This indicated that this species of bacteria has a potential to produce sugars<br />

for industrial needs.<br />

1835 | UMT UNDERGRADUATE RESEARCH DAY 2018

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