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Chemical Study of Malaysian Stingless Bee Propolis: Heterotrigona itama<br />

Nor Farah Diyana Binti Abdul Razak<br />

Supervisor: Dr. Ahmad Nazif bin Aziz<br />

Bachelor of Science (Chemical Sciences)<br />

School of Fundamental Science<br />

Stingless bee propolis has been used since ancient time as curing agent for various<br />

diseases. However, there is lack of chemical study on Malaysian stingless bee propolis.<br />

In this project, a chemical study on Heterotrigona itama propolis was conducted. The<br />

propolis was extracted by using methanol, hexane, dichloromethane, and ethyl<br />

acetate. Phytochemical screening showed the presence of phenols, tannins,<br />

flavonoids, steroids, terpenoids, alkaloids, and glycosides. Methanol and ethyl acetate<br />

extracts showed cytotoxicity against MCF-7 breast cancer cell lines with IC50 of 29<br />

µg/mL and 30 µg/mL respectively. Ethyl acetate extract showed the most potent<br />

cytotoxicity against SKUT-1 fibroid cancer cell lines with IC50 of 10 µg/mL. Ethyl acetate<br />

extract was chosen for isolation and purification process by using column<br />

chromatography and thin layer chromatography. Two compounds were successfully<br />

isolated and their structures were elucidated by using various spectroscopic<br />

techniques such as nuclear magnetic resonance, infrared, ultraviolet, and mass<br />

spectrometry.<br />

427 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018

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