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Identification of Imidazolinone Herbicide-Resistant Of Induced Mutant
Aerobic Rice
Hazurin Akmal Binti Husein
Supervisor: Prof Dr. Aziz B Ahmad
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Rice, Oryza sativa L is cultivated in wide range of environments and consist of many
varieties. Drought is an abiotic stress that reduces agricultural productivity. As varieties
with stress tolerance are introduced, issues with weed and herbicide management
arise. To improve rice production, non-transgenic approach are introduced by inducing
mutagenesis. The objective in this study is to identify the imidazolinone herbicides
resistant line in F2 generation of putative mutant aerobic rice and to determine the
point mutation in acetolactate synthase (ALS) gene. Imidazolinone herbicide-tolerant
aerobic rice are induced with potent chemical mutagen, sodium azide (NaN3). To
detect the herbicide tolerant line in F2 generation, the plant are treated with
imidazolinone herbicide. Total mortality occurs on the plant and detection of possible
point mutation at the ALS sequence which is the site of action of herbicide are
compared with Clearfield* seed and aerobic rice IURON 18 seed shows no mutation
occurs.
316 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018