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The Effect of Dietary Supplements on the Hematology of Oreochromis sp.
Wan Nursyahida binti Wan Abd Ariff
Supervisor: Dr Nor Omaima binti Harun
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Oreochromis sp. is a high demand food fish. Dietary supplements were used improve
the quality of the fish. The quality was observed through the improvement of the
immune system of the fish by determining the direct leukocyte count and differential
leukocyte count of the fish. The dietary supplements that had been used were; Eisenia
foetida, Pleurotus sajor-caju and Nepenthes gracilis. These supplements were added
into commercial pellet to produce new pellets. Head kidney, spleen and peritoneal
fluid were obtained from the fishes, every 7 days. Samples were prepared as cell
suspension before counted under hemocytometer. The differential cell count used
Giemsa stain for smear preparation. The result showed that Pleurotus sajor-caju gives
a significant effect (p < 0.05) towards the direct leukocyte count in spleen and head
kidney. Differential leukocyte count gives high percent of lymphocyte. Thus, the best
diet supplement for fish immunity can be concluded as Pleurotus sajor-caju.
385 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018