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Mode of Cell Death in Mcf-7 Cells Treated With Chalcone Derivatives
Nurul Shazwani Mohd NorHadi Shah
Supervisor: Dr. Fatimah Hashim
Co-supervisor: Assoc. Prof.Dr. Wan Mohd Khairul Wan Mohamed Zin
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Three different chalcone derivatives which are nitro, trifluomethyl and
cyanochalconehave been synthesised and were evaluated for their cytotoxicity
potential onMCF-7 cells. Based on dose-response on MCF-7 cells,nitro and
trifluoromethylchalconecompounds shows active range with IC50 values 14.75 and
13.75 µg/ml respectivelybut bit higher than tamoxifen (positive control) with IC50 value
6.40 µg/mLwhile cyanochalcone is inactive range with no IC50 value. Moreover, both
active compounds have triggered morphological changes to the MCF-7 cells as
revealed by light microscope such as cell shrinkage, rounded and clumping.It’s also
induced apoptosis asshown in AO/PI staining method with cell blebbing. Other
apoptosis indicatorthat can been seenis phosphatidylserine exposure that where
determined by using Annexin V staining method. In conclusion, nitro and
trifluoromethylchalcone compounds have proved to causeapoptosis in MCF-7 cells.
333 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018