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Occurrence of Histamine Producing Bacteria in Thunnus Tongol Treated<br />

With Garcinia Atroviridis<br />

Nor Ashikin binti Mohd Radzi<br />

Supervisor: Assoc. Prof Dr. Muhd Danish Daniel bin Abdullah<br />

Bachelor of Applied Science (Fisheries)<br />

School of Fisheries and Aquaculture Sciences<br />

Histamine poisoning is the highest food safety concern from fish consumption. Longtail<br />

tuna were purchased from Tok Jembal area, Malaysia to determine the occurrence of<br />

histamine producing bacteria in Thunnus tonggol by using Garcinia atroviridis leaves<br />

extract. The leaves extract was used in this study as it is a natural remedy that does<br />

not contain side effect of its using for seven days. The results at the initial showed<br />

that the high concentration of extracts indicated reduction of bacteria count for both<br />

PCA (Log CFU ⁄ g = 6.02) and Niven Agar (Log CFU ⁄ g 0.88). Then, the count turn for<br />

both PCA to increase at Day 1 which are 6.12, 1.62 and 1.58 while for Niven Agar are<br />

1.36, 0.57 and 0.31. The same pattern can be seen at other days. The histamine<br />

producing bacteria was identified by using Vitek system. The outcomes of the result<br />

were Morganella morganii with the probability of 93% and 96%.<br />

613 | UMT UNDERGRADUATE RESEARCH DAY 2018

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