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Therapeutic Substances in Kaffir Lime Leaves toward Cyprinus Carpio to
Bacterial Infection
Muhammad Taqiyuddin Norazman
Supervisor: Dr Sharifah Noor Emilia Syed Jamil Fadaak
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture
The Kaffir lime leaves extract that has been sprayed into commercials feed with certain
concentration can fight the disease onto Cyprinus carpio caused by the Aeromonas
hydrophila. This experiment was conducted to study the effect of Kaffir lime leaves
extract towards Cyprinus carpio against bacterial infection and the best concentration
used to fight them. A. hydrophila was cultured in Tryptic Soy Broth for 24 h at 30°c in
incubator shaker for 4 day straight to obtain concentration of 10^7CFU/ml. Infect the
C. carpio with A. hydrophila and randomly divided into group of 10 fish each tank,
conducted in triplicate. Fed C. carpio with commercials feed that has been sprayed
with essential oil with different concentrations. Fed them twice a day, started in the
morning at 0800 and evening at 1700 respectively. Mortality rates in the control tank
was the highest and show the clinical signs caused by Aeromonas hydrophila. As a
conclusion, Kaffir lime leaves extract had the potential as a probiotic that can fight
disease caused by the Aeromonas hydrophila.
568 | UMT UNDERGRADUATE RESEARCH DAY 2018