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Identification of Microalgae Isolated from Polychaetes
Aishah Naqiah Binti Zainuddin
Supervisor: Dr. Malinna binti Jusoh
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Microalgae are photosynthetic microorganisms that can be found in marine and
freshwater environment. Microalgae also are found in polychaetes and most probably
from the diet. Hence, this study was conducted to isolate and identify the microalgae
from polychaetes by using 18s rDNA gene. The polychaetes were sampled at
mangrove area Pantai Kelanang, Morib, Selangor, Malaysia and cultured on F/2 media
until single colony was obtained. DNA was extracted from the colony and served as a
template for Polymerase Chain Reaction (PCR) by using universal EuKA and EuKB
primers. A putative DNA band of 1500 base pair was cut and purified before sending
for sequencing. Based on the sequence from BLAST, it showed that Surirella sp. has
highest similarity. Therefore, it is important for this study to identify the microalgae
isolated from the polychaetes in order to fully understand the relationship between
microalgae and polychaetes.
354 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018