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The Effect of Diopatra Claparedii Grube, 1878 Extract On Chang Liver and
Human Primary Glioblastoma (U-87) Cell Lines
Amirah Binti Idris
Supervisor: Assoc. Prof. Dr. Wan Iryani Binti Wan Ismail
Bachelor of Science (Biological Sciences)
School of Fundamental Science
The distinctive regenerative ability of Diopatra claparedii has a potential to be
discovered as a cellular growth agent. In this study, the growth effect was investigated
in normal cells without giving any harm that can lead to cancer (abnormal cell growth).
Water extract of D. claparedii at different concentrations (0-100 μg/mL) was tested
on Chang Liver (normal cells), and Human Primary Glioblastoma (U-87) (cancer cells)
cell lines for 24, 48 and 72h. Percentage of cell viability was evaluated by [2-(4, 5-
dimethyl-2-thiazolyl)-3, 5-diphenyl-2H tetrazolium bromide] (MTT) assay kit. The
findings suggested that the extract has proliferative effect on normal cell growth when
tested at lower doses (80
μg/mL in all incubation periods. Meanwhile, it showed cytotoxic effect on cancer cells
only after 48h when treated with all concentrations. It shows that the extract can
induce cell growth without causing abnormal cell progression at low concentration
only, after 48h and 72h.
381 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018