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Sugarcane Bagasse as a Potential Substrate for Pectinase Production by
Aspergillus niger in Solid State Fermentation
Ngam Kai Sien
Supervisor: Dr. Nazaitulshila binti Rasit
Bachelor of Technology (Environment)
School of Ocean Engineering
Universiti Malaysia Terengganu
Sugarcane bagasse being generated in the sugar industry contains 28% of the
sugarcane. These wastes were used in solid state fermentation (SSF) for the
production of pectinase enzyme by using Aspergillus niger. The main purpose of this
study is to characterize and optimize the production of pectinase enzyme so that to
utilize it for the treatment of textile wastewater. The optimized condition for pectinase
production is 96 hours of fermentation time, 35°C, 10 g of substrate, 10 ml of inoculum
size and 4.5 pH value. After 10 consecutive days of batch test for textile wastewater
treatment, result shows a 27.35% reduction in total dissolved solid, 11.81% reduction
in biological oxygen demand and 90.05% reduction in chemical oxygen demand.
Overall, pectinase enzymes are able to treat textile wastewater successfully but further
research should be done to produce the enzyme in mass.
52 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2019